New Insights on Hemp Oil Enriched in Cannabidiol: Decarboxylation, Antioxidant Properties and In Vitro Anticancer Effect

This study aimed to obtain and characterize extracted hemp oil enriched in cannabidiol (CBD) by decarboxylation of cannabidiolic acid (CBDA) give new insights into its antioxidant anticancer effects. Optimization CBDA was performed, CBD contents purities were determined flash chromatography, 1H- 13C-NMR. The properties CBD-enriched investigated Fe2+ chelating activity, Fe3+ reducing power assay, O2●− scavenging HO● ability lipid peroxidation inhibitory cytotoxicity, apoptosis- oxidative stress-inducing effects on NHDF, MeWo, HeLa, HepG2 HOS cells determined. concentration increased soft optimized at 90 °C, for 1 h the resulting capable iron, free radicals inhibiting cell-free conditions. promoted NHDF proliferation up 15 µg CBD/mL, while inducing apoptosis ROS production modulating enzymes’ gene expression cancer cells, being selective osteosarcoma induced p53- ROS-independent mechanisms. demonstrated conditions normal fibroblasts’ proliferation, cells.